Table S4 Comparisons of MrgX2 concentrations between respective groups of healthy subject matter and CU patients. 13601_2020_361_MOESM7_ESM.docx (18K) GUID:?5A696DE4-7A83-4544-A4FB-3AE8D675CBCD Data Availability StatementThe datasets used Eptifibatide Acetate and/or analysed during the current study are available from your corresponding author on reasonable request. Abstract Background Mas-related G-protein coupled receptor member X2 (MrgX2) directly mediates drug-induced pseudo allergic reactions. of the T1 (T1M/Z?=?5222.18 Da). 13601_2020_361_MOESM2_ESM.png (187K) GUID:?48623362-8B9D-4E3D-8AEC-6866EEF2AE26 Additional file 3:?Fig. S3.?Titer detection diagram of human being MrgX2 rabbit polyclonal antibody. a Dot blot method to verify the potency of human being MrgX2 rabbit polyclonal purified antibody acknowledgement polypeptide. b Indirect ELISA method to verify the ability of human being MrgX2 rabbit polyclonal purified antibody to recognize natural MrgX2 protein. Students t test (nonparametric checks) was used to determine statistical significance. Data are indicated as mean??SEM from at least three independent experiments. **p?Prodigiosin sandwich ELISA for MrgX2 was established. The whole blood from healthy subjects and CU patients was used to detect MrgX2 concentrations. The use of feasibility of this MrgX2-ELISA as a clinical detection tool was explored and diagnostic purposes was assessed. Results The sandwich antibody ELISA method for MrgX2 was established with good linearity regression (R2?=?0.9910). The.