Methods for fluorophore targeting to cellular proteins can allow imaging with dyes that are smaller, brighter, and more photostable than fluorescent proteins. in multiple mammalian cell lines with diverse fluorophores including fluorescein, rhodamine, Alexa Fluor VE-821 568, ATTO 647N, and ATTO 655. Introduction The use of small-molecule fluorophores in live cells to replace fluorescent proteins… Continue reading Methods for fluorophore targeting to cellular proteins can allow imaging with
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Anthracycline chemotherapeutic providers of the topoisomerase inhibitor family are widely used
Anthracycline chemotherapeutic providers of the topoisomerase inhibitor family are widely used for the treatment of numerous tumors. PCR Immunoblotting Cells were lysed in extraction buffer (7 m urea, 10% glycerol, 10 mm Tris-HCl, pH 6.8, 1% SDS, 5 mm dithiothreitol) supplemented with Complete Protease Inhibitor Mixture (Roche Applied Science), and aliquots were resolved by SDS-PAGE,… Continue reading Anthracycline chemotherapeutic providers of the topoisomerase inhibitor family are widely used