Using phage antibody display, large libraries can be generated and screened to identify monoclonal antibodies with affinity for target antigens. 30 min at 30 C (and wash twice with 1 mL PBS. Resuspend cells in 500 L PBS and add 1 L mouse anti-V5 antibody. Incubate at RT with rotation for 1 h. Wash three… Continue reading Using phage antibody display, large libraries can be generated and screened